Hhinsch Week 6
Discovery Questions from Chapter 4
Question 1
Question 2
1 Hour:
- Gene x: Black
- Gene y: Black
- Gene z: Black
3 Hour:
- Gene x: Dim Red
- Gene y: Medium Red
- Gene z: Dim Red
5 Hour:
- Gene x: Black
- Gene y: Dim Green, almost Black
- Gene z: Dim Red
9 Hour:
- Gene x: Medium Green
- Gene y: Bright Green
- Gene z: Dim Red
Question 3
Yes Genes x and y were transcribed similarly, they both show a similar pattern which is a strike in transcription then nearing zero as time increases.
Question 4
Most spots would be yellow at the first time point because the ratio of the two cDNA’s is 1:1. The technical reason that spots show up as yellow is that there was no change in a gene’s transcription and experimental growth conditions. The yellow color technically comes from the computer placing a yellow color on the spots that are open reading frames. The biological reason for most of the spots showing up as yellow is that genes are expressed in both transcriptomes.
Question 5
Over the course of the experiment TEF4 was repressed. I hypothesize that the change of expression due to lack of glucose is the fact that TEF4 needs glucose in order to release the eEF1A from the ribosomal complex.
Question 6
The TCA cycling genes would be induced if the glucose supply is running out because pyruvate is shifted away from the acetaldehyde and toward oxalacetate and into the TCA cycle which would cause the glucose supply of the TCA cycling genes to be repressed, thus inducing them.
Question 7
In order to ensure genes for enzymes in a common pathway are induced or repressed simultaneously the genome could use the same pathway in order to have the genes react in concert.
Question 8
I would predict that the color of the spots that represented the glucose repressed genes at the end of the sequence would also be labeled red.
Question9
I would predict that the color of the spots that represented the glucose repressed genes at the end of the sequence would also be labeled red.
Question 10
Using the microarray data, we could truly verify that we deleted TUP1 or over expressed YAP1 in the experiments by looking to see if there are in fact spots that are labeled red rather than green or yellow.
Acknowledgments
- I worked with Zachary Van Ysseldyk via text communication in order to complete the deliverables of this assignment.
- I worked with Corinne Wong in person in order to complete the deliverables of this assignment.
- While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source.Hhinsch (talk) 22:54, 9 October 2017 (PDT)
References
- LMU BioDB 2017. (2017). Week 6. Retrieved October 9, 2017, https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_6
- https://brightspace.lmu.edu/d2l/le/content/36331/viewContent/350003/View
- https://brightspace.lmu.edu/d2l/le/content/36331/viewContent/350004/View
- https://www.yeastgenome.org/locus/S000001564#expression
Assignments
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 14
Week 15
Hayden's Individual Journal Entries
hhinsch Week 1
hhinsch Week 2
hhinsch Week 3
hhinsch Week 4
hhinsch Week 5
hhinsch Week 6
hhinsch Week 7
hhinsch Week 8
hhinsch Week 9
hhinsch Week 10
hhinsch Week 11
hhinsch Week 12
hhinsch Week 14
hhinsch Week 15
Page Desiigner Deliverables Page
Class Journal Entries
Class Journal Week 1
Class Journal Week 2
Class Journal Week 3
Class Journal Week 4
Class Journal Week 5
Class Journal Week 6
Class Journal Week 7
Class Journal Week 8
Class Journal Week 9
Class Journal Week 10
Page Desiigner
