Vpachec3 Week 2

There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
- In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
- The genetic code is read in the 5’ to 3’ direction.
- Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
- By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.

Complementary Strand

3’-gcatacgattatggtacaagcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’

N-ter- R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L- C-ter

5’-guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’

N-ter-V-C-STOP-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F-C-ter

5’-uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’

N-ter-Y-A-N-T-M-F-R-V-STOP-P-S-R-Q-F-R-W-R-H-F-C-ter

5’-uaaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3’

N-ter-STOP-N-A-A-S-G-T-G-G-W-V-I-R-G-G-T-W-Y-STOP-H-T -C-ter

5’-aaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3’

N-ter-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I- C-ter

5’-aaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3’

N-ter- K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y- C-ter

+1, -2 and -3