Jwoodlee Week 2
From LMU BioDB 2015
5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3' 3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
- There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence.
mRNA sequence(top): 5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3' mRNA sequence(bottom): 3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'
+1 5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'
Amino Acids:Nter-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-Cter
+2 5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'
Amino Acids:Nter-V-C-Cter
+3 5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'
Amino Acids:Nter-Y-A-N-T-M-F-R-V-Cter
-1 5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg-3'
Amino Acids: None, the first codon is a stop codon
-2 5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua cg-3'
Amino Acids:Nter-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-Cter
-3 5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac g-3'
Amino Acids:Nter-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-Cter
- Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
- +1, -2, and -3 are open reading frames.
Electronic Lab Notebook
Week 2 Assignment:
- Wrote out complementary DNA strand.
- Transcribed the DNA strand into two different mRNA strands.
- Broke up the mRNA strands in to 6 different reading frames 3 different frames per mRNA strand.
- Translated mRNA codons to amino acids using the mRNA codon translation chart.
- Wrote down the open reading frames at the end of the question.
BIOL 367, Fall 2015, User Page, Team Page
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