Jkuroda Week 2

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand:

Original DNA strand:
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’

Complementary DNA strand:
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

mRNA strand:
5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3'

Complementary mRNA strand:
3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'

Six reading frames:

+1                       5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'
Corresponding amino acids:   R   M   L   I   P   C   S   A   Y   N   P   A   A   S   S   A   G   G   I   L

+2                       5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'
Corresponding amino acids:     V   C   STOP

+3                       5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'
Corresponding amino acids:      Y   A   N   T   M   F   R   V   STOP

-1                       5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg-3'
Corresponding amino acids: STOP  N   A   A   S   G   T   G   G   W   V   I   R   G   T   W   Y   STOP

-2                       5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua cg-3'
Corresponding amino acids:     K   M   P   P   A   E   L   A   A   G   L   Y   A   E   H   G   I   S   I

-3                       5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac g-3'
Corresponding amino acids:      K   C   R   Q   R   N   W   R   L   G   Y   T   R   N   M   V   L   A   Y

+1, -2 and -3 do not contain stop codons, so they are open reading frames

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