Sinorhizobium meliloti

Team 4

Main Paper: The composite genome of the legume symbiont Sinorhizobium meliloti. Science 293, 668–672.

Personnel

Stephen Louie

Project Manager, Quality Assurance

slouie4 at lion.lmu.edu

1 LMU Drive MSB 5194

Los Angeles, CA 90045

Lauren Magee

GenMAPP Expert

lmagee1 at lion.lmu.edu

1 LMU Drive MSB-5258,

Los Angeles, CA 90045

Mitchell Petredis

Coding Supervisor

mrpetredis at gmail dot com

mpetredi at lion dot lmu dot edu

Loyola Marymount University

1 LMU Drive MSB-5957

Los Angeles, CA 90045-2659

Miles Malefyt

GenMAPP Coordinator

milesm@malefyt.com

mmalefyt@lion.lmu.edu

8416 Campion drive

Westchester, CA 90045

cell: 831-236-5402

Mmalefyt (talk) 10:31, 31 October 2013 (PDT)

Micro Array papers

1. [Construction and validation of a Sinorhizobium meliloti whole genome DNA microarray: genome-wide profiling of osmoadaptive gene expression.]
2. [Development of a lab-made microarray for analyzing the genetic diversity of nitrogen fixing symbionts Sinorhizobium meliloti and Sinorhizobium medicae.]
3. [Dual RpoH sigma factors]
4. [Osmotic upshift elicited by salt and sucrose]
5. [Expression in nitrogen stress response between wild and mutant types under high and low nitrogen availability]
6. [Development of a lab-made microarray for analyzing the genetic diversity of nitrogen fixing symbionts Sinorhizobium meliloti and Sinorhizobium medicae]
7. [A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti]

Paper Descriptions

DNA microarrays are used to characterize the Sinorhizobium population. Three distinct DNA regions, or loci, were investigated. These three loci, called “IGSGAB”, “IGSEXO” and “IGSRKP”. The wildtypes acted as the controls while the treatment involved the use of microarray probes. No indication of replications were given.

sRNAs are used for gene expression in bacteria. sRNAs were screened for in Sinorhizobium meliloti through the use of microarray hybridizations. It was unapparent whether replications were used in the nature of this experiment.

Citations

Xavier Bailly, Gilles Béna, Vanina Lenief, Philippe de Lajudie, Jean-Christophe Avarre, Development of a lab-made microarray for analyzing the genetic diversity of nitrogen fixing symbionts Sinorhizobium meliloti and Sinorhizobium medicae, Journal of Microbiological Methods, Volume 67, Issue 1, October 2006, Pages 114-124, ISSN 0167-7012, http://dx.doi.org/10.1016/j.mimet.2006.03.006. (http://www.sciencedirect.com/science/article/pii/S0167701206000662)

Jan-Philip Schlüter, Jan Reinkensmeier, Svenja Daschkey, Elena Evguenieva-Hackenberg, Stefan Janssen, Sebastian Jänicke, Jörg D Becker, Robert Giegerich, Anke Becker, A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti BMC Genomics. 2010; 11: 245. Published online 2010 April 17. doi: 10.1186/1471-2164-11-245 PMCID: PMC2873474 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873474/

More microarray papers can be found at mpetredi Week 10