Mpetredi Week 2

Mitchell Petredis

The Genetic Code

• Original DNA Strand

5’-cgt atg cta ata cca tgt tcc gcg tat aac cca gcc gcc agt tcc gct ggc ggc att tta-3’

• Complementary DNA Strand

3’-gca tac gat tat ggt aca agg cgc ata ttg ggt cgg cgg tca agg cga ccg ccg taa aat-5’

Reading Frames

Note: numbered list indicates shift in reading frames. Direction (Forward / Reverse) is indicated by section name.

• Forward (Positive)

1. Arg-Met-Leu-Ile-Pro-Cys-Ser-Ala-Tyr-Asn-Pro-Ala-Ala-Ser-Ser-Ala-Gly-Gly-Ile-Leu
2. Val-Cys-STOP-Tyr-His-Val-Pro-Arg-Ile-Thr-Gln-Pro-Pro-Val-Pro-Leu-Ala-Ala-Phe
3. Tyr-Ala-Asn-Thr-Met-Phe-Arg-Val-STOP-Pro-Ser-Arg-Gln-Phe-Arg-Trp-Arg-His-Phe

• Reverse (Negative)

1. STOP-Asn-Ala-Ala-Ser-Gly-Thr-Gly-Gly-Trp-Val-Ile-Arg-Gly-Thr-Trp-Tyr-STOP-His-Thr
2. Lys-Met-Pro-Pro-Ala-Glu-Leu-Ala-Ala-Gly-Leu-Tyr-Ala-Glu-His-Gly-Ile-Ser-Ile
3. Lys-Cys-Arg-Gln-Arg-Asn-Trp-Arg-Leu-Gly-Tyr-Thr-Arg-Asn-Met-Val-Leu-Ala-Tyr

• Open Reading Frames (ORFs)
  • +1
  • -2
  • -3