Ajvree Week 2

• Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgt-atg-cta-ata-cca-tgt-tcc-gcg-tat-aac-cca-gcc-gcc-agt-tcc-gct-ggc-ggc-att-tta-3’

3'-gca-tac-gat-tat-ggt-aca-agg-cgc-ata-ttg-ggt-cgg-cgg-tca-agg-cga-ccg-ccg-taa-aat-5'

• There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.

+1 strand:  R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L
+2 strand:  V-C-STOP-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F
+3 strand:  Y-A-N-T-M-F-R-V-STOP-P-S-R-Q-F-R-W-R-H-F

-1 strand:   STOP-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-STOP-H-T
-2 strand:   K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I
-3 strand:   K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y

Open reading frames: Strands +1, -2, and -3

Ajvree (talk) 09:34, 6 September 2013 (PDT)

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