HDelgadi Week 2
Hilda Delgadillo
Original DNA Strand
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
Complementary Strand
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’
RNA Strands-Transcription
+1, +2, +3
5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
-1, -2, -3
3’-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5’
Amino Acids-Translation
5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
+1: (N – Terminus) RMLIPCSAYNPAASSAGGIL (C – Terminus)
+2: (N – Terminus) VC“stop”YHVPRITRPPVPLAAF (C – Terminus)
+2: (N – Terminus) VC“stop”YHVPRIT Q PPVPLAAF (C – Terminus) ( Corrected Translation )
I made the mistake of a point mutation since the genetic code for Arginine, CGA, is very similar to Glutamine, CAG, the right answer.
+3: (N – Terminus) YANTMFRV“stop”PSRQFRWPHF (C – Terminus)
+3: (N – Terminus) YANTMFRV“stop”PSRQFRW R HF (C – Terminus) ( Corrected Translation )
I made the mistake of a point mutation since the genetic code for Proline, CCG, is very similar to Arginine, CGG, the right answer.
3’-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5’
-1: (N – Terminus) “stop”NAASGTGGWVIRGTWY“stop”HT (C – Terminus)
-2: (N – Terminus) KMPPAELAAGLYAEHGISI (C – Terminus)
-3: (N – Terminus) KCRQRNWRLGYTRNMVLAY (C – Terminus)
Open Reading Frames are +1, -2, and -3
