Plate control of protein CLN1 of Saccharomyces cerevisiae from an experiment analyzing protein localization and abundance changes during DNA replication stress (Tkach, J. M, et al., 2012).
1 ATGAACCACT CAGAAGTGAA AACTGGGTTA ATTGTCACTG CAAAGCAGAC ATATTACCCA
61 ATTGAATTGT CCAATGCAGA ACTACTAACT CATTACGAAA CCATACAGGA ATATCACGAG
121 GAAATCTCTC AAAATGTGCT GGTCCAATCT TCCAAGACAA AACCAGACAT AAAATTGATC
181 GATCAGCAAC CGGAGATGAA TCCTCATCAA ACTAGAGAAG CCATAGTAAC ATTTTTGTAT
241 CAACTTTCAG TGATGACTAG AGTAAGTAAT GGTATCTTCT TCCACGCTGT CAGGTTCTAC
301 GATCGCTATT GCTCTAAGAG AGTAGTGTTA AAGGACCAAG CTAAACTAGT TGTAGGCACC
361 TGCCTTTGGT TAGCGGCCAA AACTTGGGGA GGGTGCAACC ATATTATAAA CAACGTCTCC
421 ATCCCCACAG GTGGTAGGTT TTATGGTCCC AATCCTAGAG CTCGTATTCC ACGCCTTTCT
481 GAATTGGTTC ATTATTGCGG CGGGTCCGAT TTATTCGATG AATCAATGTT CATTCAAATG
541 GAAAGACATA TCTTGGATAC TCTGAACTGG GACGTTTATG AGCCCATGAT TAATGACTAC
601 ATTTTAAACG TTGACGAAAA TTGTTTGATA CAATATGAAC TTTACAAAAA CCAGTTACAA
661 AATAACAATA GCAACGGCAA AGAATGGTCC TGTAAGAGAA AGTCACAATC TTCTGACGAC
721 AGTGATGCCA CAGTGGAAGA ACATATCAGT AGTTCACCGC AAAGTACTGG ACTAGATGGC
781 GATACAACTA CCATGGATGA AGATGAAGAA CTAAATTCCA AAATTAAATT GATAAATTTG
841 AAAAGATTCT TAATTGATCT GAGCTGTTGG CAATACAACT TGCTTAAATT CGAATTATAC
901 GAAATATGCA ATGGTATGTT TTCTATAATA AACAAATTCA CTAATCAAGA TCAAGGCCCT
961 TTTCTCTCTA TGCCCATTGG TAATGATATA AACTCAAACA CTCAAACGCA GGTATTCAGC
1021 ATTATCATCA ATGGCATAGT CAATTCTCCC CCATCTTTAG TCGAAGTTTA TAAGGAGCAA
1081 TATGGTATAG TACCTTTCAT ATTACAAGTA AAAGATTATA ACTTGGAATT ACAAAAGAAA
1141 CTGCAACTGG CCTCTACAAT AGACCTAACC AGAAAAATTG CTGTCAATTC TCGTTACTTT
1201 GACCAAAATG CCTCTTCATC ATCAGTTTCT TCTCCAAGCA CATATTCTTC GGGAACCAAT
1261 TATACTCCAA TGCGAAACTT CAGTGCACAA TCAGACAACA GTGTTTTCAG TACTACCAAC
1321 ATTGACCATT CATCGCCGAT CACCCCTCAC ATGTACACTT TTAATCAGTT TAAAAACGAA
1381 AGTGCTTGTG ACAGTGCCAT AAGCGTAAGC AGTCTACCTA ATCAAACCCA AAATGGTAAC
1441 ATGCCATTAT CAAGCAATTA TCAGAATATG ATGCTAGAAG AAAGGAATAA AGAGAATAGA
1501 ATTCCCAACT CATCATCCGC TGAAATACCA CAACGTGCCA AATTTATGAC GACTGGTATA
1561 TTCCAGAACA CAGGAGAACT TACTAATAGA GCGTCTTCGA TATCGCTATC GTTAAGGAAC
1621 CACAATAGCT CTCAACTGTG A
1 MNHSEVKTGL IVTAKQTYYP IELSNAELLT HYETIQEYHE EISQNVLVQS SKTKPDIKLI
61 DQQPEMNPHQ TREAIVTFLY QLSVMTRVSN GIFFHAVRFY DRYCSKRVVL KDQAKLVVGT
121 CLWLAAKTWG GCNHIINNVS IPTGGRFYGP NPRARIPRLS ELVHYCGGSD LFDESMFIQM
181 ERHILDTLNW DVYEPMINDY ILNVDENCLI QYELYKNQLQ NNNSNGKEWS CKRKSQSSDD
241 SDATVEEHIS SSPQSTGLDG DTTTMDEDEE LNSKIKLINL KRFLIDLSCW QYNLLKFELY
301 EICNGMFSII NKFTNQDQGP FLSMPIGNDI NSNTQTQVFS IIINGIVNSP PSLVEVYKEQ
361 YGIVPFILQV KDYNLELQKK LQLASTIDLT RKIAVNSRYF DQNASSSSVS SPSTYSSGTN
421 YTPMRNFSAQ SDNSVFSTTN IDHSSPITPH MYTFNQFKNE SACDSAISVS SLPNQTQNGN
481 MPLSSNYQNM MLEERNKENR IPNSSSAEIP QRAKFMTTGI FQNTGELTNR ASSISLSLRN
541 HNSSQL*
CLN1 is a gene involved in protein coding found on the chromosome XIII (CLN1 | SGD, 2017). It encodes for a G1 cyclin which regulates the cell cycle meaning they activate CDKs at the correct times within the cell cycle. It can be found within the nucleus and the cytoplasm of the cell (CLN1 | SGD, 2017). To further characterize CLN1, the genomic sequence is NC_001145.3 and the name of the mRNA and protein Reference Sequences are NM_001182706.1 and NP_013926.1 respectively (CLN1 | SGD, 2017). Another primary way to identify the gene is through the locus name or tag: YMR199W. The processes CLN1 is involved in includes the cell cycle, cell division, and regulation of serine/threonine kinase activity. Lastly, to discuss the physical characteristics, the protein length was measured to be 546 a.a., the isoelectric point to be 5.62, and the molecular weight to be 62038.2 Da (CLN1 | SGD, 2017).
The two of us met outside of class and worked on the project together. Eddie handled the website, while Antonio handled the content.
Made edits based off the feedback received from Dr. Dondi and Dr. Dalquist.
~Eddie Azinge and Antonio Porras