Why We Should All Love SPT15!

What are the SPT15 gene's functions?

Gene Summary
SPT15 is a TATA binding protein that binds TFIID to a promoter, recruiting other transcription factors
to contribute to the preinitiation complex (PIC) (UniProt 2017). It can bind to the promoter either by binding directly
to the TATA box, or by recruitment from other transcription factors (SGD 2017). This means it can bind to the
promoter with or without the TATA box, making it an important factor in initiating transcription. This
allows SPT15 to have an important role in regulating gene expression by RNA polymerase II. SPT15
and PIC can be slowed down to regulate transcription (UniProt 2017).

More You Should Know


  1. Standard Name: SPT15.

    (SGD 2017)

    2. Systematic Name: YER148W.

    (Ensembl 2017)

    Name Description: SuPpressor of Ty insertions (SPT)

    (SGD 2017)



  2. SGD ID:S000000950

    (SGD 2017)

    3. NCBI gene ID:856891

    (NCBI 2017)

    Ensemble References ID's from SGD and UniProt

    (Ensembl 2017)

    UniProt ID:P13393

    (UniProt 2017)


    YeastMine (SGD)

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    UniProt

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    DNA Sequence
    001 ATGGCCGATG AGGAACGTTT AAAGGAGTTT AAAGAGGCAA ACAAGATAGT GTTTGATCCA
    061 AATACCAGAC AAGTATGGGA AAACCAGAAT CGAGATGGTA CAAAACCAGC AACTACTTTC
    121 CAGAGTGAAG AGGACATAAA AAGAGCTGCC CCAGAATCTG AAAAAGACAC CTCCGCCACA
    181 TCAGGTATTG TTCCAACACT ACAAAACATT GTGGCAACTG TGACTTTGGG GTGCAGGTTA
    241 GATCTGAAAA CAGTTGCGCT ACATGCCCGT AATGCAGAAT ATAACCCCAA GCGTTTTGCT
    301 GCTGTCATCA TGCGTATTAG AGAGCCAAAA ACTACAGCTT TAATTTTTGC CTCAGGGAAA
    361 ATGGTTGTTA CCGGTGCAAA AAGTGAGGAT GACTCAAAGC TGGCCAGTAG AAAATATGCA
    421 AGAATTATCC AAAAAATCGG GTTTGCTGCT AAATTCACAG ACTTCAAAAT ACAAAATATT
    481 GTCGGTTCGT GTGACGTTAA ATTCCCTATA CGTCTAGAAG GGTTAGCATT CAGTCATGGT
    541 ACTTTCTCCT CCTATGAGCC AGAATTGTTT CCTGGTTTGA TCTATAGAAT GGTGAAGCCG
    601 AAAATTGTGT TGTTAATTTT TGTTTCAGGA AAGATTGTTC TTACTGGTGC AAAGCAAAGG
    661 GAAGAAATTT ACCAAGCTTT TGAAGCTATA TACCCTGTGC TAAGTGAATT TAGAAAAATG

    (SGD 2017)







    Protein Sequence
    001 MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT
    061 SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK
    121 MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG
    181 TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM

    (UniProt 2017)


  3. Gene Function
    SPT15 functions in transcription. It binds the transcription factor complex, TFIID, to a promoter.
    It can bind to the promoter regardless of the presence of the TATA box. This makes it a very
    important factor in forming the preinitiation complex (PIC).

    (UniProt 2017)


  4. Differences in Sources
    All of our sources contained the same basic identifiers for the gene; however, both SGD and UniPort contained more information. Ensembl and< NCBI had basic identifiers and a map of the gen from seconday sources. SGD and UniProt had a more detailed summary of the gene's function.They also contained detailed infromation on interctions, taxonomy, sequences, and processes. Much of the information provided came from separate links
    on the pages. SGD provided the most information; including it's function, process, history, and
    other interactions. All of the pages organized their information into sections and provided maps
    and diagrams of the gene and/or it's region.

  5. Why We Chose SPT15
    After both Corinne and we perused the selection of genes at length, I am saddened to say that
    nothing struck us with particular interest. After some discussion, we decided to choose one at
    random. Surprisingly, We are not unhappy with the gene that we ended up with. This is probably
    because we never felt uncomfortable with the material throughout the entire experiemnt.

Photo Gallery


This is a picture of transcription!
This diagram shows the beggining of transcription where SPT15 would bind to TFIID.

(Wikipedia 2017)

This is a picture of transcription!
This diagram shows a gene "switched on" and a gene "switched off." Transcription is only possible when the gene is switched on, which allows for transcription factors. SPT15 will bind to these transcription factors, initiating transcription, and thus, regulating gene expression.

(Wikimedia 2017)

Acknowledgements

References