Standard Name: HSF1
Systematic name: YGL073W
Name Description: Named because it codes for one of two Heat Shock transcription Factors
1 ATGAATAATG CTGCAAATAC AGGGACGACC AATGAGTCAA ACGTGAGCGA TGCTCCCCGT
61 ATTGAGCCTT TACCAAGCTT GAATGATGAT GACATTGAAA AAATCTTACA ACCGAACGAT
121 ATCTTTACGA CCGATCGTAC CGATGCAAGT ACTACATCTT CCACAGCCAT TGAAGATATT
181 ATTAACCCCT CATTGGATCC GCAGTCAGCA GCATCGCCGG TTCCTTCTTC CTCTTTTTTC
241 CATGACTCAA GGAAACCTTC CACCAGTACA CATTTAGTAA GGAGAGGTAC TCCATTGGGA
301 ATTTACCAAA CCAATCTATA CGGTCACAAT AGCAGAGAAA ATACTAATCC TAATAGTACA
361 TTATTATCTT CTAAGTTACT CGCGCATCCA CCAGTTCCTT ATGGGCAAAA TCCCGATTTA
421 CTACAACATG CTGTGTACAG GGCACAGCCG TCAAGTGGAA CCACTAACGC GCAACCGCGC
481 CAAACCACAA GAAGATATCA ATCCCATAAA TCACGGCCTG CATTTGTTAA TAAACTATGG
541 AGCATGTTAA ACGATGATTC TAATACGAAA CTTATACAGT GGGCGGAGGA TGGAAAATCT
601 TTTATTGTCA CGAATAGGGA GGAATTTGTG CACCAAATTT TACCAAAATA TTTTAAACAT
661 TCCAATTTCG CTTCCTTTGT AAGACAATTG AACATGTATG GATGGCATAA AGTTCAAGAT
721 GTCAAGTCAG GATCAATTCA AAGTAGTTCA GATGATAAGT GGCAATTTGA AAATGAAAAC
781 TTCATTAGAG GTAGAGAAGA TTTGCTGGAA AAAATAATCA GGCAGAAAGG TTCCTCCAAT
841 AACCATAATA GCCCTAGTGG TAACGGTAAT CCAGCGAATG GTAGCAACAT CCCTCTGGAC
901 AATGCCGCAG GAAGTAATAA TAGCAATAAT AACATCAGTA GTAGTAATTC ATTTTTTAAC
961 AATGGTCATT TATTGCAGGG TAAAACACTA AGATTAATGA ACGAAGCGAA TCTTGGAGAT
1021 AAGAATGATG TCACCGCGAT TTTGGGGGAA TTAGAGCAAA TAAAATATAA CCAGATTGCA
1081 ATTTCCAAAG ATTTACTAAG AATAAACAAA GATAATGAGT TATTATGGCA AGAGAATATG
1141 ATGGCCAGGG AAAGACATAG AACCCAACAG CAAGCCTTGG AAAAAATGTT CAGATTCTTG
1201 ACATCTATAG TCCCACACTT AGATCCCAAA ATGATTATGG ACGGGCTGGG AGATCCGAAA
1261 GTTAATAATG AAAAGCTAAA CAGTGCGAAT AACATTGGGT TAAATCGCGA CAACACAGGC
1321 ACTATAGATG AACTAAAATC CAACGATTCT TTCATAAACG ATGATCGTAA TTCTTTCACC
1381 AATGCTACAA CCAACGCCCG TAATAACATG AGTCCCAACA ATGATGACAA TAGTATTGAC
1441 ACCGCTAGCA CTAATACCAC CAACAGAAAG AAAAATATAG ATGAAAACAT CAAAAATAAC
1501 AACGACATAA TTAATGACAT TATATTTAAT ACCAACCTTG CCAACAATCT CAGCAATTAC
1561 AATTCCAACA ATAATGCTGG CTCGCCAATA AGGCCCTATA AACAAAGATA TCTTTTGAAA
1621 AATAGAGCCA ATTCCTCGAC ATCGAGTGAG AATCCAAGCC TAACGCCCTT TGATATCGAA
1681 TCTAATAATG ACCGCAAAAT TTCAGAAATT CCTTTTGATG ACGAAGAAGA AGAAGAAACG
1741 GATTTTAGGC CTTTTACCTC GCGAGATCCT AATAACCAAA CGAGTGAAAA CACTTTTGAT
1801 CCAAACAGAT TTACGATGCT CTCTGATGAT GATTTAAAAA AAGATTCTCA TACCAATGAC
1861 AATAAACACA ACGAAAGTGA TCTTTTTTGG GACAACGTAC ATAGAAATAT AGACGAACAA
1921 GATGCAAGAC TCCAGAACTT GGAAAATATG GTTCACATAC TTTCTCCTGG ATATCCTAAT
1981 AAGTCGTTCA ACAACAAAAC TTCCTCGACA AACACTAATT CCAATATGGA AAGTGCTGTC
2041 AACGTTAATA GCCCTGGTTT CAACTTACAG GATTATTTAA CTGGAGAGTC TAATTCCCCC
2101 AATTCTGTTC ATTCTGTTCC CTCCAATGGC AGCGGCTCCA CACCGTTGCC CATGCCAAAT
2161 GATAATGACA CCGAGCACGC AAGTACAAGT GTCAATCAAG GCGAAAATGG AAGCGGATTA
2221 ACGCCCTTCC TCACGGTAGA TGATCACACA CTAAACGACA ATAACACTAG TGAGGGAAGT
2281 ACAAGGGTGT CCCCCGATAT AAAGTTCAGC GCCACTGAAA ACACTAAAGT GAGTGATAAC
2341 CTGCCAAGCT TTAATGACCA CAGTTATTCC ACCCAGGCCG ACACGGCGCC CGAGAACGCT
2401 AAGAAAAGAT TTGTGGAGGA AATACCGGAA CCGGCTATAG TCGAAATACA GGACCCGACA
2461 GAGTACAACG ATCACCGCCT GCCCAAACGA GCTAAGAAAT AG
1 MNNAANTGTT NESNVSDAPR IEPLPSLNDD DIEKILQPND IFTTDRTDAS TTSSTAIEDI
61 INPSLDPQSA ASPVPSSSFF HDSRKPSTST HLVRRGTPLG IYQTNLYGHN SRENTNPNST
121 LLSSKLLAHP PVPYGQNPDL LQHAVYRAQP SSGTTNAQPR QTTRRYQSHK SRPAFVNKLW
181 SMLNDDSNTK LIQWAEDGKS FIVTNREEFV HQILPKYFKH SNFASFVRQL NMYGWHKVQD
241 VKSGSIQSSS DDKWQFENEN FIRGREDLLE KIIRQKGSSN NHNSPSGNGN PANGSNIPLD
301 NAAGSNNSNN NISSSNSFFN NGHLLQGKTL RLMNEANLGD KNDVTAILGE LEQIKYNQIA
361 ISKDLLRINK DNELLWQENM MARERHRTQQ QALEKMFRFL TSIVPHLDPK MIMDGLGDPK
421 VNNEKLNSAN NIGLNRDNTG TIDELKSNDS FINDDRNSFT NATTNARNNM SPNNDDNSID
481 TASTNTTNRK KNIDENIKNN NDIINDIIFN TNLANNLSNY NSNNNAGSPI RPYKQRYLLK
541 NRANSSTSSE NPSLTPFDIE SNNDRKISEI PFDDEEEEET DFRPFTSRDP NNQTSENTFD
601 PNRFTMLSDD DLKKDSHTND NKHNESDLFW DNVHRNIDEQ DARLQNLENM VHILSPGYPN
661 KSFNNKTSST NTNSNMESAV NVNSPGFNLQ DYLTGESNSP NSVHSVPSNG SGSTPLPMPN
721 DNDTEHASTS VNQGENGSGL TPFLTVDDHT LNDNNTSEGS TRVSPDIKFS ATENTKVSDN
781 LPSFNDHSYS TQADTAPENA KKRFVEEIPE PAIVEIQDPT EYNDHRLPKR AKK*
The HSF1 gene is a transcription factor, meaning it is a gene that regulates the expression of other genes. Specifically, HSF1 regulates genes in response to stresses such as heat, pH imbalance, starvation, or other stressors. HSF1 regulates a large range of genes which are involved in things like protein folding, metabolism, protein transport, etc. in order to protect the cell during while in stress. This gene is in yeast, but it is also in other organisms including mammals, birds, and plants. In yeast, the removal of this gene is fatal and mutation of it usually results in defects of the cell.This gene encodes a transcription factor in response to stress, especially heat shock related stress. This gene is necessary for the yeast’s survival. In cells which are not under stress, the gene is not active, but if the cell becomes under stresses such as heat, pH, or starvation, it becomes active to regulate the transcription of other genes, which work to protect the organism.
The Saccharomyces Genome Database by far had the most information on this gene compared to the other three databases. The SGD website says that HSF1 is inactive in normal conditions, but the UniProt website says that this gene is necessary for normal growth. The NCBI Gene website has the same information as the SGD site, with little or no differences. The Ensembl website also does not have very much information on the function of the gene, what it did have was the same as the SGD website. I could not find anything under “Gene Expression” on this website.
The SGD website presented the information very clearly. The tabs at the top as well as the side menu made it easy to navigate each section of the website to find the information I was looking for. The UniProt website was also pretty simple, it had a list on the side where I could choose what I wanted to see, which also made finding information easy. The NCBI website also had a table of contents where I could click to which section i wanted, making it easy to find their information on the gene’s function. The Ensembl website also had a menu, but it was slightly less simple. Some of the categories in the menu did not come up with any results when I clicked them.
We chose this gene by reading through the blog posts on the SGD website. They mentioned that this one was used in cases of heat stress. I think it’s interesting that there are genes that are prepared to regulate other genes in response to high levels of heat or other stressors, so I chose this one. My partner agreed that this was interesting and we both wanted to learn more.